Cultured in RPMI1640 (Life Technologies GIBCO BRL, Grand Island, NY, USA
Cultured in RPMI1640 (Life Technologies GIBCO BRL, Grand Island, NY, USA) supplemented with ten heatinactivated fetal calf serum (FCS, Hyclone, Logan, Utan, USA) and antibiotics (penicillin 100 U/ml, streptomycin one…