Product Name :
Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody [PS01-18]
Predicted band size :
Observed band size :
Synonyms:
Ataxia telangiectasia and Rad3 related antibody Ataxia telangiectasia and Rad3-related protein antibody ATR antibody ATR_HUMAN antibody FCTCS antibody FRAP Related Protein 1 antibody FRAP-related protein 1 antibody FRP1 antibody MEC1 antibody MEC1 mitosis entry checkpoint 1 homolog antibody 展开
Function :
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates ‘Ser-139’ of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. Positively regulates the restart of stalled replication forks following activation by the KHDC3L-OOEP scaffold complex.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phosphopeptide corresponding to residues surrounding Thr1989 of human ATR.
Species reactivity:
Human
Verify application:
WB, IF-Cell
Coupling :
unconjugated
Clone number:
PS01-18
RRID:
AB_3072310
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.BuySilver(I) carbonate Aliquot store at -20℃.Formula of 3-Bromo-2-methylpyrazolo[1,5-a]pyridine Avoid repeated freeze / thaw cycles.PMID:33560165
Storage buffer solution:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 301 kDa
Positive control :
HeLa cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 20 hours.